Individual research ESR 10

Zebrafish models of vascular development and disease


Objectives:

  • Characterize at least one existing zebrafish model for a mutation known to cause a vascular anomaly in humans (CCM, HHT, VM, BRBN)
  • Characterize the phenotype of the zebrafish model with intra-vital microscopy, developmental genetics, and cell biological methods
  • Perform a detailed molecular characterization of the zebrafish mutants by whole-mount in situ hybridizations, immunohistochemical methods, and RNASeq
  • Elucidate potentially important signaling pathways using pharmacological intervention, genetic reporter lines and genetic interaction studies with other zebrafish mutants in relevant pathways

Expected Results:

  • Characterization of an existing disease model in the zebrafish embryo;
  • Elucidation of the molecular pathways misregulated in that model;
  • Comparison of misregulated gene profiles with data available from patients;
  • Identification of a pharmacological intervention strategy.

Secondments:

  • AstraZeneca
  • de Duve Institute
  • Max Planck Institute for Heart and Lung Research
  • LLS Rowiak LaserLab Solutions GmbH